| 光谱,波谱法研究铜锌超氧歧化酶与氯化钴（Ⅱ）,组氨酸钴（？… |
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| 引用本文： | 胡皆汉,郑学仿.光谱,波谱法研究铜锌超氧歧化酶与氯化钴（Ⅱ）,组氨酸钴（？…[J].光谱学与光谱分析,1999,19(3):304-309. |
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| 作者姓名： | 胡皆汉 郑学仿 |
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| 作者单位： | [1]中国科学院大连化学物理研究所 [2]大连大学化学化工系 |
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| 摘 要： | 我们首次发现的铜锌超氧歧化酶（Ｃｕ２Ｚｎ２ＳＯＤ）与氨基酸等发生直接相互作用的现象，是一种前人未研究过的重要的生化新现象。在此新发现的基础上，本文用ＩＣＰ，ＶＩＳ，ＮＭＲ和酶活性测定方法，又从不同角度拓展研究了Ｃｕ２Ｚｎ２ＳＯＤ酶与两类不同化合物，
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| 关 键 词： | 铜锌超氧歧化酶 氯化钴 SOD 组氨酸钴 相互作用 |
Study on the interaction of Cu2Zn2SOD with CoCl2, Co(His)n by spectral and magnetic resonance analysis] |
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| J Hu,X Zheng,Y Xu,G Cheng,Y Zhao.Study on the interaction of Cu2Zn2SOD with CoCl2, Co(His)n by spectral and magnetic resonance analysis][J].Spectroscopy and Spectral Analysis,1999,19(3):304-309. |
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| Authors: | J Hu X Zheng Y Xu G Cheng Y Zhao |
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| Institution: | Dalian Institute of Chemical Physics, Academia Sinica, 116023 Dalian. |
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| Abstract: | Direct interaction of Cu2Zn2SOD with inorganic metal compound (CoCl2) and organic metal compound Co(II)(His)n] was studied by ICP, VIS, NMR and measurement of enzyme activity. It has been found that in aqueous solution, there exists a direct interaction of the metal ions of the active center in the metalloenzyme (Cu2Zn2SOD) with external added CoCl2, Co(His)n. As a result, part of the metal ions in metalloenzyme were replaced and the corresponding metalloenzyme derivatives were produced and the catalytic activity of enzyme were affected. Moreover, the interaction of Co(II)(His)n with Cu2Zn2SOD is stronger and quicker, than that of CoCl2 with this enzyme, as well as the Co(II) from Co(II)(His)n is easier to enter the active center of enzyme. |
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