| Mn（Ⅱ）,Co（Ⅱ）与HSA相互作用的荧光光谱研究 |
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| 引用本文： | 查丹明,申泮文.Mn（Ⅱ）,Co（Ⅱ）与HSA相互作用的荧光光谱研究[J].光谱学与光谱分析,1999,19(6):788-791. |
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| 作者姓名： | 查丹明 申泮文 |
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| 作者单位： | 广西师范大学生物无机化学研究所化学系 |
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| 基金项目： | 国家自然科学基金，广西十百千人才基金，广西高校自然科学基金 |
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| 摘 要： | 用荧光光谱法研究了生理ｐＨ和等离子点（ｐＨ＝５．３０）时Ｍｎ（Ⅱ）、Ｃｏ（Ⅱ）与ＨＳＡ的相互作用。根据Ｆｏｒｓｔｅ非辐射能量转移理论，得到了不同ｐＨ时Ｍｎ（Ⅱ）、Ｃｏ（Ⅱ）在ＨＳＡ中的第一强结合位置与Ｔｒｐ－２１４残基间的距离。这一结果远大于文献报道值，根据Ｍｎ（Ⅱ）、Ｃｏ（Ⅱ）在ＨＳＡ中的结合部位及ＨＳＡ的畴结构对这一显著差异进行了讨论。
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| 关 键 词： | 人血清白蛋白 锰 钴 荧光光谱 相互作用 |
Study on the interaction of human serum albumin and Mn (II), Co (II) by fluorescence method] |
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| D Zha,S Li,Y Yang,C Tu,H Liang,P Shen.Study on the interaction of human serum albumin and Mn (II), Co (II) by fluorescence method][J].Spectroscopy and Spectral Analysis,1999,19(6):788-791. |
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| Authors: | D Zha S Li Y Yang C Tu H Liang P Shen |
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| Institution: | Institute of Bioinorganic Chemistry, Department of Chemistry, Guangxi Normal University, 541004 Guilin. |
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| Abstract: | The interaction of Mn (II), Co(II) and HSA has been studied by fluorescence method at pH 7.4 and 5.3. Based on Forste non-radiative energy transfer theory, the distance between Trp-214 residue of HSA and the first strong binding site to Mn (II), Co (II) in HSA was determined, and it is much larger than that of reference. This notable different result has been discussed according to the binding site of Mn (II), Co (II ) and domain structure in HSA. |
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| Keywords: | Human serum albumin Fluorescence spectra Non radiative engergy transfer Binding site |
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