Effect of sucrose on BSA denatured aggregation at high concentration studied by the iso-conversional method and the master plots method |
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| Authors: | X Cao Z Wang X Yang Y Liu C Wang |
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| Institution: | (1) College of Chemistry and Molecular Science, Wuhan University, Wuhan, 430072, Hubei, China;(2) College of Life Science, Wuhan University, Wuhan, 430072, Hubei, China |
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| Abstract: | The kinetics of protein thermal transition is of a significant interest from the standpoint of medical treatment. The effect
of sucrose (0–15 mass%) on bovine serum albumin denatured aggregation kinetics at high concentration was studied by the iso-conversional
method and the master plots method using differential scanning calorimetry. The observed aggregation was irreversible and
conformed to the simple order reaction. The denaturation temperature (T
m), the kinetic triplets all increased as the sucrose concentration increased, which indicated the remarkable stabilization
effect of sucrose. The study purpose is to provide new opportunities in exploring aggregation kinetics mechanisms in the presence
of additive. |
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| Keywords: | denatured aggregation DSC iso-conversional method master plots method |
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