Rapid determination of ranitidine in human plasma by high-performance liquid chromatography |
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Authors: | M. A. Campanero A. Lopez-Ocariz E. García-Quetglás B. Sádaba A. de la Maza |
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Affiliation: | (1) Departmento de Farmacología Clínica, Clínica Universitaria de Navarra, Universidad de Navarra, 31008 Pamplona, Spain;(2) Laboratorios CINFA S. A., Pamplona, Spain |
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Abstract: | Summary An HPLC method has been developed for the quantification of rantidine in plasma for pharmacokinetic studies. Metoclopramide was used as internal standard. The method uses a simple and rapid sample clean-up procedure involving single-step extraction with organic solvent to extract ranitidine from plasma. After evaporation and reconstitution the samples are chromatographed on a 250 mm×4 mm base-stable reversed-phase column with 0.05 M ammonium acetate-acetonitrile, 75∶25 (v/v) as mobile phase and UV detection at 313 nm. The calibration graph was linear for quantities of ranitidine between 10 and 2000 ng mL−1. Intra- and inter-dayCV did not exceed 11.64%. The quantitation limit was 10 ng mL−1 for human plasma. The applicability of this method for pharmacokinetic studies of ranitidine after oral administration are described. Approximately 90 samples can be processed in 24 h. |
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Keywords: | Column liquid chromatography Ranitidine Plasma Pharmacokinetics |
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