Effects of BaCl_2 on slow vacuolar ion channels on radish by patch-clamp

Effects of BaCl2 on slow vacuolar ion channels on radish by patch-clamp YANG Pin (杨 频) & ZHANG Liping (张丽平) Institute of Molecular Science, Shanxi University, Taiyuan 030006, China Correspondence should be addressed to Yang Pin (email: yangpin@sxu.edu.cn)

Effects of BaCl2 on slow vacuolar ion channels on radish by patch-clamp

The effects of BaCl2 on slow vacuolar (SV) currents of radish are studied by using the whole-vacuolar patch-clamp recording mode. The Ca2+-dependent SV channel can be activated by cytosolic Ca2+. When 1 mmol/L BaCl2 is added intopipette solution, SV currents are suppressed remarkably. Then adding BaCl2 of different concentrations into the bath solution, SV currents reflect different effects. The results show that BaCl2 with a lower concentration (＜ 3 mmol/L) promotes the channel currents and the currents are saturated when BaCl2 concentrations are between 1mmol/L and 1 mmol/L, but BaCl2 with higher concentration (≥ 3 mmol/L) inhibits SV currents.