Rapid chemical ligation of oligonucleotides by the Diels-Alder reaction |
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| Authors: | El-Sagheer Afaf H Cheong Vee Vee Brown Tom |
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| Affiliation: | School of Chemistry, University of Southampton, Highfield, Southampton, UK SO17 1BJ. ahes@soton.ac.uk |
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| Abstract: | Extremely fast and efficient Diels-Alder chemical ligation of furan and maleimide oligonucleotides has been carried out in aqueous buffer. It was possible to ligate three oligonucleotides simultaneously in a controlled manner with the aid of a complementary splint. The templated reactions proceeded within 1 min at room temperature whereas non-templated reactions were slow and incomplete. Rapid and clean methods of DNA ligation such as the one demonstrated here have potential uses in biology and nanotechnology. |
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