| CdTe量子点-罗丹明B荧光共振能量转移猝灭法测定金银花中的微量铜 |
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| 引用本文： | Wang XM,Ye C,Li CG,Hu YZ. CdTe量子点-罗丹明B荧光共振能量转移猝灭法测定金银花中的微量铜[J]. 光谱学与光谱分析, 2011, 31(2): 448-451. DOI: 10.3964/j.issn.1000-0593(2011)02-0448-04 |
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| 作者姓名： | Wang XM Ye C Li CG Hu YZ |
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| 作者单位： | 1. 中国药科大学分析化学教研室,江苏,南京,210009
2. 中国药科大学分析化学教研室,江苏,南京,210009;药物质量与安全预警教育部重点实验室,江苏,南京,210009 |
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| 基金项目： | 国家自然科学基金项目(30873197)资助 |
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| 摘 要： | 研究CdTe量子点(供体)和罗丹明B(受体)之间荧光共振能量转移的最佳条件,建立了荧光共振能量转移猝灭法测定金银花中微量铜的新方法.采用十六烷基三甲基溴化铵,在pH 6.00的Tris-HCl缓冲液中,Cu2+能对能量转移体系中罗丹明B荧光峰强猝灭从而测定铜的含量.Cu2+浓度在1.3×10-4～3.1×10-2 μg...
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| 关 键 词： | 量子点 荧光共振能量转移 荧光猝灭 金银花 铜 |
Study on the fluorescence resonance energy transfer between CdTe QDs and rhodamine B and its application to the determination of trace amount of copper in flos lonicerae japonicae |
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| Wang Xiao-Mei,Ye Chao,Li Chen-Gui,Hu Yu-Zhu. Study on the fluorescence resonance energy transfer between CdTe QDs and rhodamine B and its application to the determination of trace amount of copper in flos lonicerae japonicae[J]. Spectroscopy and Spectral Analysis, 2011, 31(2): 448-451. DOI: 10.3964/j.issn.1000-0593(2011)02-0448-04 |
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| Authors: | Wang Xiao-Mei Ye Chao Li Chen-Gui Hu Yu-Zhu |
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| Affiliation: | WANG Xiao-mei1,YE Chao1,LI Chen-gui1,HU Yu-zhu1,2 1.Department of Analytical Chemistry,China Pharmaceutical University,Nanjing 210009,China 2.Key Laboratory of Drug Quality Control and Pharmacovigilance Affiliated to Ministry of Education,China |
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| Abstract: | The present work is to find the optimum condition for fluorescence resonance energy transfer between CdTe QDs(donor) and rhodamine B(acceptor) and form a new method to detect copper in Flos Lonicerae Japonicae.In tris-HCl buffer solution at pH 6.00,by employing cetyltrimethylammonium bromide,Cu2+ can quench the fluorescence intensity of BRB and detect the concentration of Cu2+.Results show that a linear relationship could be established between the quenched fluorescence intensity of RhB and the concentratio... |
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| Keywords: | Quantum dots FRET Fluorescence quenching Flos Lonicerae Japonicae Copper |
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