| 南极海洋芳香烃低温降解微生物的拉曼光谱分析 |
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| 引用本文： | Wang YB,Miao JL,He BJ,Liang Q,Liu FM,Zheng Z. 南极海洋芳香烃低温降解微生物的拉曼光谱分析[J]. 光谱学与光谱分析, 2011, 31(2): 418-421. DOI: 10.3964/j.issn.1000-0593(2011)02-0418-04 |
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| 作者姓名： | Wang YB Miao JL He BJ Liang Q Liu FM Zheng Z |
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| 作者单位： | 1. 国家海洋局第一海洋研究所,山东,青岛,266061;国家海洋局海洋生物活性物质重点实验室,山东,青岛,266061
2. 广西科学院,广西,南宁,530007 |
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| 基金项目： | 国家自然科学基金项目(40876107); 国家海洋局青年科学基金项目(2010122); 广西自然科学基金项目(0832077); 青岛市科技发展项目(09-1-3-4-jch)资助 |
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| 摘 要： | 激光镊子拉曼光谱技术可以实现在自然状态下对单个细胞或细胞器较长时间的观察研究.应用激光镊子拉曼光谱技术实时观察南极微生物低温降解芳香烃过程中单个南极细菌的细胞生长和胞内生物大分子的动态变化过程,收集、分析其拉曼光谱,结果发现:单细胞的拉曼光谱反映了其细胞内部的生命物质组成,南极动球菌 NJ41 和希瓦氏菌 NJ49 生...
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| 关 键 词： | 激光镊子拉曼光谱技术 南极微生物 低温降解 芳香烃 |
Laser tweezers Raman spectroscopy analysis of cold-adapted aromatic hydrocarbons-degradating strains isolated from Antarctic Sea |
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| Wang Yi-Bin,Miao Jin-Lai,He Bi-Juan,Liang Qiang,Liu Fang-Ming,Zheng Zhou. Laser tweezers Raman spectroscopy analysis of cold-adapted aromatic hydrocarbons-degradating strains isolated from Antarctic Sea[J]. Spectroscopy and Spectral Analysis, 2011, 31(2): 418-421. DOI: 10.3964/j.issn.1000-0593(2011)02-0418-04 |
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| Authors: | Wang Yi-Bin Miao Jin-Lai He Bi-Juan Liang Qiang Liu Fang-Ming Zheng Zhou |
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| Affiliation: | WANG Yi-bin1,2,MIAO Jin-lai1,HE Bi-juan3,LIANG Qiang1,LIU Fang-ming1,ZHENG Zhou1,2 1.First Institute of Oceanography,State Oceanic Administration,Qingdao 266061,China 2.Key Lab of Marine Bioactive Substances,China 3.Guangxi Academy of Sciences,Nanning 530007,China |
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| Abstract: | Laser tweezers Raman spectroscopy can help with observing and studying individual cells or organelles in a natural state for a relatively long period.In the present experiment,Laser tweezers Raman spectroscopy(LTRS) was used as a tool to report physiological metabolism such as cells growth and nucleic acid,proteins,lipid and glucose of a single active cold-adapted Aromatic hydrocarbons-degradating strains isolated from Antarctic Sea.After the Raman spectrum was collected and analyzed,the findings are as fol... |
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| Keywords: | Laser tweezers Raman spectroscopy(LTRS) Antarctica microorganisms Low-temperature biodegradation Polycyclic aromatic hydrocarbons(PAHs) |
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