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| 油橄榄叶中总黄酮含量测定方法探讨 | |
| 引用本文： | Zheng YY,Li C,Feng SL,Huang XY,Liu YF,Chen XF,Di DL. 油橄榄叶中总黄酮含量测定方法探讨[J]. 光谱学与光谱分析, 2011, 31(2): 547-550. DOI: 10.3964/j.issn.1000-0593(2011)02-0547-04 |
| 作者姓名： | Zheng YY Li C Feng SL Huang XY Liu YF Chen XF Di DL |
| 作者单位： | 1. 兰州大学药学院,甘肃,兰州,730000;中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃,兰州,730000 2. 中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃,兰州,730000;五邑大学应用物理与材料学院分析测试中心,广东,江门,529020 3. 兰州大学药学院,甘肃,兰州,730000 4. 中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃,兰州,730000 5. 中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室和甘肃省天然药物重点实验室,甘肃,兰州,730000;中国科学院研究生院,北京,100049 |
| 基金项目： | 中国科学院“百人计划”项目和中国科学院“科技支甘”项目资助 |
| 摘 要： | 建立以芦丁为对照品的油橄榄叶中总黄酮含量的测定方法。采用Al(NO3)3-NaNO2-NaOH和AlCl3两种显色法测定油橄榄叶中总黄酮的含量,并分别考察了两种方法对测定结果的影响。结果表明,AlCl3显色法适宜于油橄榄叶总黄酮的含量测定。该法操作简便,线性关系良好,准确度、重复性和稳定性较好,可用于油橄榄叶总黄酮含量测定,并为中药和天然产物中总黄酮的含量测定提供参考。 |
| 关 键 词： | 油橄榄叶 总黄酮 紫外-可见分光光度法 AlCl3显色法 |
Study on the content determination of total flavonoids in Olea europaea L. leaves |
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| Zheng Yuan-Yuan,Li Chen,Feng Shi-Lan,Huang Xin-Yi,Liu Yong-Feng,Chen Xiao-Fen,Di Duo-Long. Study on the content determination of total flavonoids in Olea europaea L. leaves[J]. Spectroscopy and Spectral Analysis, 2011, 31(2): 547-550. DOI: 10.3964/j.issn.1000-0593(2011)02-0547-04 | |
| Authors: | Zheng Yuan-Yuan Li Chen Feng Shi-Lan Huang Xin-Yi Liu Yong-Feng Chen Xiao-Fen Di Duo-Long |
| Affiliation: | ZHENG Yuan-yuan1,2,LI Chen2,3,FENG Shi-lan1,HUANG Xin-yi2,LIU Yong-feng2,4,CHEN Xiao-fen2,DI Duo-long1,2 1.Lanzhou University Pharmaceutical College,Lanzhou 730000,China 2.Key Laboratory of Chemistry of Northwestern Plant Resources & Key Laboratory for Natural Medicine of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,China 3.Instrumental Analysis & Research Center,School of Applied Physics & Material Engineering,Wuyi University,Jiangmen 529020,China 4.Gra... |
| Abstract: | A quantitative determination method of the total flavonoids in Olea europaea L.leaves using rutin as a standard substance was developed.Two coloration systems including Al(NO3)3-NaNO2-NaOH and AlCl3 were both used.The latter was selected as the optimal determination method by comparing with the results.The main interference of the matrix was discussed.The result showed that the AlCl3 coloration method is simple,accurate and reproducible with good linear relationship,and it can be looked as a reference metho... |
| Keywords: | Olea europaea L.leaves Total flavonoids UV-Vis spectrophotometry AlCl3 coloration |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |