超高效液相色谱法测定湿巾中的三氯羟基二苯醚

采用超高效液相色谱法(UPLC)检测湿巾中的三氯羟基二苯醚含量.湿巾样品加甲醇超声提取,离心过滤后上机,采用甲醇-水(体积比为8∶2)为流动相,Phenomenex C18色谱柱分离,在波长280 nm下检测,外标法定量.结果表明:方法在0.50~100.00 mg/L质量浓度范围内线性良好,相关系数为0.999 1,检出限为0.30 mg/kg,方法定量限为10.0 mg/kg,RSD为2.3%,加标回收率为90.50%~93.50%.方法前处理简便快捷,准确度、精密度、稳定性好,检测结果准确可靠.

Determination of Triclosan in Wet Wipes by Ultra-Performance Liquid Chromatography

Triclosan in wet wipes was determined by ultra-performance liquid chromatography(UPLC). The sample was extracted with methanol, centrifuged, filtrated and detected using 80% MeOH and 20% H₂O as the mobile phase. The flow rate was 0.2 mL/min. Triclosan was separated on a Phenomenex C₁₈ chromatographic column and detected at 280 nm. The correlation coefficient of this method in 0.50~100.00 mg/L was 0.999 1. The determination limit was 0.30 mg/kg with a method quantification limit of 10.0 mg/kg. The RSD was 2.3% and the recovery was 90.50%~93.50%. This method is simple in sample preparation, it has good precision, accuracy and stability.