G蛋白在杨树气孔运动中的作用

群众杨叶片下表皮经过不同浓度的G蛋白激活剂霍乱毒素(CTX)处理后，在扫描电镜下观察了气孔开度的变化，并用透射电镜结合X-射线能谱显微分析技术，对保卫细胞内的K^ 、Cl^-含量进行了研究。结果表明：CTX能促进气孔关闭，作用强度随CTX浓度的增加而增强。伴随着气孔关闭，保卫细胞液泡和细胞质中的K^ 、Cl^-含量都明显下降，而细胞壁中的K^ 、Cl^-含量增加。这提示G蛋白可能通过对保卫细胞内K^ 、Cl^-的调节作用而参与了气孔运动过程。

Roles of G-protein in The Regulation of Stomatal Movement in Poplar

Epidermis of P.popularis was treated by CTX. The changes of stomatal aperture were observed by SEM. K +, Cl - contents in vacuole, cytoplasm, cell wall of guard cells were analysed by TEM X-ray microanalysis. The results indicated that CTX promoted stomatal closing. When stomatal closure, K +,Cl - contents decreased obviously in vacuole and cytoplasm, but increased in cell wall of guard cells. It is suggested that G-protein maybe involved in the regulation of stomatal movement.