婴幼儿奶瓶中迁移双酚A的高效液相色谱-电喷雾串联质谱检测方法研究

建立了婴幼儿奶瓶中双酚A(BPA)迁移量的高效液相色谱-电喷雾串联质谱(HPLC-ESI-MS/MS)测定方法。奶瓶食品模拟浸泡液经过弗罗里硅土玻璃层析柱净化,高效液相色谱分离,采用选择反应性监测模式(SRM)检测。以一级质谱得到的准分子离子m/z 227作为母离子,进行二级质谱(MS2)分析。选择MS2的碎片离子m/z 212、133、93定性确证,m/z 212作为定量离子定量。实验优化了质谱条件,并对二级质谱碎裂机理和特征离子进行了研究。测定结果的相对标准偏差不大于8.2%(n=7),回收率在87.7%～105%之间;检出限为2μg/L,能够满足欧盟、美国等对奶瓶中双酚A的限制要求。该法已成功应用于婴幼儿奶瓶中BPA迁移量的测定。

STUDY ON THE MIGRATION OF BISPHENOL A FROM BABY BOTTLES BY LIQUID CHROMATOGRAPHY - TANDEM MASS SPECTROMETRY

A method was established for the determination of bisphenol A （BPA） migration from baby bottles by high performance liquid chromatography - tandem mass spectrometry interfaced with electro - spray ionization （ HPLC - ESI - MS/MS）. The migration of BPA from baby bottles was cleaned up on Florisil columns, and analyzed by HPLC - ESI - MS/MS. The conditions of LC -ESI -MS/MS were optimized. The parention of （ - ） ESI MS was m/z 277, and it＇s daughter ions of MS/MS m/z 212,133,93 were selected as qualitative. The m/z 212 was selected as quantitative of BPA. The BPA recovery was in the range of 87.7% - 105% and the RSD was no more than 8.2% （ n = 7）. The limit of determination was 2 μg/L and meet the requirements of EU, US. The method has been applied to the determination of BPA migration from baby bottles.