Controlled encapsulation of single-cells into monodisperse picolitre drops |
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Authors: | Edd Jon F Di Carlo Dino Humphry Katherine J Köster Sarah Irimia Daniel Weitz David A Toner Mehmet |
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Institution: | Massachusetts General Hospital, BioMEMS Resource Center, Charlestown, MA, USA. |
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Abstract: | Encapsulation of cells within picolitre-size monodisperse drops provides new means to perform quantitative biological studies on a single-cell basis for large cell populations. Variability in the number of cells per drop due to stochastic cell loading is a major barrier to these techniques. We overcome this limitation by evenly spacing cells as they travel within a high aspect-ratio microchannel; cells enter the drop generator with the frequency of drop formation. |
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