Simple method for the assay of linezolid in Brain Heart Infusion broth by high-performance liquid chromatography

A simple HPLC method was developed and validated for quantification of linezolid in Brain Heart Infusion (BHI) broth. Eperezolid was employed as internal standard and the sample pre-treatment procedure was simple. Calibration standards ranged from 0.05 to 16 mg/L. Accuracy was within 7.8% and reproducibility (RSD) was less than 8.3%. Recovery was approximately 100% at all concentrations examined. Linezolid was stable in the autosampler insert for at least 24 h at ambient temperature and in BHI for 72 h at 37 degrees C. This assay is rapid and ideal for analysis of a large number of samples.