Retention of histidine-containing peptides on a nickel affinity column |
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Authors: | Kozlov Igor A Kermani Bahram G Melnyk Peter C Barker David L Zhao Chanfeng Hachmann John P Lebl Michal |
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Affiliation: | Illumina, Inc., San Diego, CA 92121, USA. |
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Abstract: | The retention of histidine-containing peptides in immobilized metal-affinity chromatography is studied using several hundred modeled peptides. Retention is driven primarily by the number of histidine residues; however, the amino acid composition in the immediate vicinity plays a significant role. Specifically, the arginine and tryptophan content has to be taken into consideration. During the course of this study, an alternative tag that can be used similarly to a polyhistidine tag is discovered. |
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