Simultaneous spectrophotometric determination of pyrantel pamoate and febantel in pharmaceutical preparations using partial least-squares regression |
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Authors: | M S Piantavini F L D Pontes L B Cerqueira P G Peralta-Zamora R Pontarolo |
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Institution: | 1. Laboratório de Controle de Qualidade, Departamento de Farmácia Universidade Federal do Paraná, Av. Pref.Lothário Meissner, 632, 80210-170, Curitiba-PR, Brasil 2. Laboratório de Química Ambiental e de Materiais, Departamento de Química, Curitiba-PR, Brasil
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Abstract: | The multivariate spectroscopic determination of the combination of pyrantel pamoate (PP) and febantel (FB) in pharmaceutical preparations was carried out by partial least-squares regression. The UV absorbance spectra of standard mixtures of PP and FB were measured at concentrations ranging from 5.76–11.52 and 6–12 μg/mL, respectively, in a diluent solution of methanol and acetonitrile. The best model was selected with variable selection (280–320, 380–400 nm), 2 latent variables and mean centered. External validation was performed using 13 different mixtures, which provided prediction errors lower than 1%. The method was validated in accordance with international and Brazilian guidelines. This method was successfully employed to quantify the drug content in two different pharmaceutical formulations (solid — capsules, liquid — oral suspensions) with good prediction capacity. Furthermore, its advantages include the use of simple and accessible reagents and equipment, and a low operating cost. |
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