Liquid chromatography of some steroid hormones in aqueous-organic,micellar, and cyclodextrin mobile phases |
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| Authors: | E G Sumina S N Shtykov O N Sorokina V Z Uglanova |
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| Institution: | 1. Institute of Chemistry, Saratov State University, ul. Astrakhanskaya 83, Saratov, 410012, Russia
2. Department of Microbiology, Biotechnology, and Chemistry, Saratov State Agricultural University, Teatral’naya pl. 1, Saratov, 410012, Russia
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| Abstract: | The retention of hydrocortisone, progesterone, dexamethasone, and prednisolone is studied by HPLC and thin-layer chromatography using aqueous-organic, micellar, and cyclodextrin mobile phases. It is demonstrated that micellar and cyclodextrin mobile phases improve the efficiency and selectivity of the chromatographic separation of the indicated hormones compared to aqueous-organic eluents. By comparing the numbers of theoretical plates, the heights equivalent to a theoretical plate, and resolution it was found that micellar mobile phases are more efficient than cyclodextrin ones and can be used for work with Sorbfil plates. |
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