| 漆酶的活性测定及催化单取代Fc—R氧化反应 |
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| 引用本文： | 季立才,胡培植,汤贵生,刘惠英.漆酶的活性测定及催化单取代Fc—R氧化反应[J].武汉大学学报(理学版),1996(4). |
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| 作者姓名： | 季立才 胡培植 汤贵生 刘惠英 |
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| 作者单位： | 武汉大学生命科学学院 化学系，江西南昌化工原料厂 |
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| 摘 要： | 在二乙二醇单丁醚（ＤＧＢＥ）／磷酸盐缓冲液体系中，以二茂铁（Ｆｃ—Ｈ）为底物，用分光光度法测定湖北毛坝漆树漆酶的活性无副产物干扰．该方法简便、准确、易行．考察了漆树漆酶对２９个单取代二茂铁衍生物（Ｆｃ—Ｒ）和左旋多巴的催化氧化反应，发现了漆酶１４个新的底物，并讨论了它们的构效关系
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| 关 键 词： | 酶活性，漆树漆酶，分光光度法，二茂铁，底物 |
THE DETERMINATION OF LACCASE ACTIVITY AND ITS CATALYTIC OXIDATION TO MONOSUBSTITUTE FERROCENE DERIVATIVES |
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| Ji Licai,Hu Peizhi,Tang Guisheng,Liu Hueying.THE DETERMINATION OF LACCASE ACTIVITY AND ITS CATALYTIC OXIDATION TO MONOSUBSTITUTE FERROCENE DERIVATIVES[J].JOurnal of Wuhan University:Natural Science Edition,1996(4). |
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| Authors: | Ji Licai Hu Peizhi Tang Guisheng Liu Hueying |
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| Abstract: | The determination of activity of Rhus vernicifera laccase from Hubei Maoba have not interference of by product using ferrocene as substrate in the system of diethylene glycol monobutyl ether (DGBE)/phosphate butter by spectrophotometric method.The method is a simple,convenient,accurate and easy.It was investigated that Rhus vernicifera laccase catalyzed oxidation of 29 monosubstitute ferrocene derivatives and left dopa.New 14 substrates of laccase were found,and the relationship of theirs structure and function is discussed. |
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| Keywords: | enzyme activity Rhus vernicifera laccase spectrophotometric method ferrocene substrate |
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