| 猪胰脂肪酶的固定化及其在有机相中催化丁酸甲酯和正丁醇的酯交换反应研究 |
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| 引用本文： | 谢志东,何炳林,吕宪禹.猪胰脂肪酶的固定化及其在有机相中催化丁酸甲酯和正丁醇的酯交换反应研究[J].高等学校化学学报,1994(9). |
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| 作者姓名： | 谢志东 何炳林 吕宪禹 |
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| 作者单位： | 南开大学高分子化学研究所，南开大学生物系 |
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| 摘 要： | 考察了大孔苯乙烯－二乙烯苯交联共聚物的交联度、致孔剂量及胺化试剂对载体固定化猪胰脂肪酶的影响。选择出一种最佳载体对猪胰脂肪酶进行固定化，对比了自由酶和固定化酶在有机相中催化丁酸甲酯和正丁醇的酯交换反应。结果表明，酶经固定化后催化反应活力比自由酶提高近１倍。
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| 关 键 词： | 聚苯乙烯，猪胰脂肪酶，固定化，有机相，酯交换反应 |
Immobilization of Porcine Pancreas Lipase and Its Catalyzed Transesterification Reaction of Methyl Butyrate and 1-Butanol in Organic Systems |
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| XIE Zhi-Dong,HE Bing-Lin.Immobilization of Porcine Pancreas Lipase and Its Catalyzed Transesterification Reaction of Methyl Butyrate and 1-Butanol in Organic Systems[J].Chemical Research In Chinese Universities,1994(9). |
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| Authors: | XIE Zhi-Dong HE Bing-Lin |
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| Abstract: | Series of macroporous cross-linked polymers of styrene with divinylbenzene and their functionalization products were synthesized. Effects of degree of cross-linking,amount of pore-generating agent and different aminating agents on immobilization of porcine pancreas lipase(PPL)were studied.The best carrier was selected.Transesterification of methyl butyrate with 1-butanol was catalyzed by the free and immobilized PPL,it was found that the catalytic activity of immobilized PPL in organic systems was two times as high as that of the free PPL. |
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| Keywords: | Polystyrene Porcine pancreas lipase Immobilization Organic systems Transesterification |
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