超高效液相色谱-四极杆串联飞行时间质谱分析黄酮类化合物及小毛茛茎叶的成分

为了探索液相色谱-质谱联用(LC-MS)技术在快速识别中药及天然产物成分中的应用,以黄酮对照品为研究前体,药用植物小毛茛为研究对象,采用超高效液相色谱/二极管阵列检测器-电喷雾四极杆串联飞行时间质谱(UPLC/DAD-ESI/Q-TOF MS)分析了黄酮类化合物同系物及同分异构体的色谱、质谱特性。结果显示:黄酮氧苷和黄酮碳苷的紫外吸收光谱及二级质谱具有显著性差异,糖苷化位置同保留时间、二级质谱碎片及相对丰度具有相关性。将该方法应用于小毛茛茎叶醇提液的分析,结合其酸水解液的分析,解析了22个黄酮醇糖苷和3个苷元。方法简便,具有可操作性。

Analysis of flavonoids and components of stems & leaves of Ranunculus Ternatus Thunb. using ultra performance liquid chromatography-quadrupole tandem time of flight mass spectrometry

In order to explore the applications of liquid chromatography-mass spectrometry (LC-MS) technology in the rapid identification of components of Chinese herbal medicines and natural products, reference flavones were used as precursors, and the stems and leaves of medicinal plant Ranunculus ternatus Thunb. were used as the objects. Ultra performance liquid chromatography with a diode array detector-electrospray ionization quadrupole tandem time of flight mass spectrometry (UPLC/DAD-ESI/Q-TOF MS) was also used to analyse the characteristics of flavonoid homologues and flavonoid isomers. The results showed that ultraviolet (UV) absorption and MS/MS spectra of C-glycosyl were distinct from O-glycosyl. There was also a correlation between glycosidation positions and the retention times, MS/MS fragments and their relative abundances. When applied it to analyse the alcohol extract of stems and leaves of Ranunculus ternatus Thunb. and their acid hydrolysis solution, 22 flavonol glycosides and 3 flavonoid aglycones were identified. The method is simple, convenient and exercisable.