| 浙江两地产索氏桃花水母核糖体小亚基rRNA基因序列分析 |
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| 引用本文： | 胡义波,姜乃澄.浙江两地产索氏桃花水母核糖体小亚基rRNA基因序列分析[J].浙江大学学报(理学版),2006,33(5):559-566. |
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| 作者姓名： | 胡义波 姜乃澄 |
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| 作者单位： | 浙江大学,生命科学学院,浙江,杭州,310028 |
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| 基金项目： | 浙江省杭州市科技局资助项目 |
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| 摘 要： | 采用PCR和DNA测序技术，对杭州与绍兴产桃花水母标本的核糖体小亚基rRNA基因进行了扩增与测序．经序列比对分析，杭州与绍兴产桃花水母的核糖体小亚基rRNA基因序列与已知索氏桃花水母的序列极为相似，相似度分别为99．61％和99．45％；与索氏桃花水母的遗传距离均为0．001；经分子系统树分析，杭州与绍兴产桃花水母与索氏桃花水母的分支置信度高达1000．4．综上分析，杭州与绍兴产桃花水母均为索氏桃花水母（Craspedacusta sowerbyi），该结论与其形态学分类相一致．
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| 关 键 词： | 索氏桃花水母 rRNA基因 核糖体小亚基 分类学 |
| 文章编号： | 1008-9497（2006）05-559-08 |
| 收稿时间： | 2005-06-21 |
| 修稿时间： | 2005年6月21日 |
Sequence analyses of small subunit ribosomal RNA genes of Craspedacusta sowerbyi from two regions in Zhejiang, China |
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| HU Yi-bo,JIANG Nai-cheng.Sequence analyses of small subunit ribosomal RNA genes of Craspedacusta sowerbyi from two regions in Zhejiang, China[J].Journal of Zhejiang University(Sciences Edition),2006,33(5):559-566. |
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| Authors: | HU Yi-bo JIANG Nai-cheng |
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| Institution: | College of Life Sciences, Zhej iang University, Hangzhou 310028, China |
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| Abstract: | The small subunit ribosomal RNA genes of Craspedacusta sp. from Hangzhou and Shaoxing were proliferated and sequenced. As a result of sequence analyses, the small subunit ribosomal RNA gene sequences of C. sp. from Hangzhou and Shaoxing are most similar to that of C. sowerbyi and the similarity percent are 99.61% and 99.45 %respectively. The pairwise genetic distances between C. sp. from Hangzhou or Shaoxing and C. sowerbyi are both 0. 001. The molecular phylogenetic tree shows 100% degree of confidence of branch between C. sp. of Hangzhou, Shaoxing, and C. sowerbyi. Summarily, C. sp. from Hangzhou and Shaoxing are C. sowerbyi. The conclusions are consistent with the results of morphological taxonomy. |
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| Keywords: | Craspedacusta sowerbyi rRNA gene small subunit ribosome taxonomy |
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