| 原花青素对照品组份的UPLC-MS及RP-HPLC分析 |
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| 引用本文： | 单岩,李永仙,郑飞云,刘春凤,李崎.原花青素对照品组份的UPLC-MS及RP-HPLC分析[J].分析试验室,2012(5):76-79. |
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| 作者姓名： | 单岩 李永仙 郑飞云 刘春凤 李崎 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室;江南大学生物工程学院酿酒科学与工程研究室 |
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| 基金项目： | 教育部新世纪人才资助计划(No.NCET-0453);江苏高校优势学科建设工程资助项目(PAPD);国家创新基金(09C26213203751);江苏省创新基金(BC2009291)资助 |
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| 摘 要： | 利用UPLC-MS和RP-HPLC方法分析了葡萄籽原花青素对照品组份。对照品使用色谱柱Zorbax Eclipse XDB-C18(4.6×250 mm,5μm),甲醇-水(含0.05%三氟乙酸)作为流动相,梯度洗脱分离后得到8种组份。依据ESI-MS选择性,使用超高效液相色谱-电喷雾质谱联用(UPLC-ESI-MS)区分各化合物组份,鉴定得到3种单体,3种二聚体,1种三聚体,1种二聚体单酯。为研究啤酒酿造用原料酒花中的原花青素提供了对照基础。
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| 关 键 词： | 原花青素 组份 RP-HPLC UPLC-MS |
Analysis of components of proanthocyanidin reference substance by UPLC-MS and RP-HPLC |
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| SHAN Yan,LI Yong-xian,ZHENG Fei-yun,LIU Chun-feng,and LI Qi.Analysis of components of proanthocyanidin reference substance by UPLC-MS and RP-HPLC[J].Chinese Journal of Analysis Laboratory,2012(5):76-79. |
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| Authors: | SHAN Yan LI Yong-xian ZHENG Fei-yun LIU Chun-feng and LI Qi |
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| Institution: | 1,2(1.Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University;2.Lab of Brewing Science and Technology,Jiangnan University,Wuxi 214122) |
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| Abstract: | Components of proanthocyanidin reference substance from grape seed extracts were analysed column by UPLC-MS and RP-HPLC.Target compounds were separated by a Zorbax Eclipse XDB-C18(4.6×250 mm,5 μm) under a gradient elution system of methanol-water(including 0.05% TFA).A UPLC-electrospray ionization-mass spectrometric(HPLC-ESI-MS) method was used to differentiate these compounds according to the selectivity of the ESI-MS.Then three monomers,three dimers,one trimer and one dimer-monoester were detected.This article provides the basis for the research of hop proanthocyanidin. |
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| Keywords: | Proanthocyanidins Components RP-HPLC UPLC-MS |
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