首页 | 本学科首页   官方微博 | 高级检索  
     


Talbot holographic illumination nonscanning (THIN) fluorescence microscopy
Authors:Yuan Luo  Vijay Raj Singh  Dipanjan Bhattacharya  Elijah Y. S. Yew  Jui‐Chang Tsai  Sung‐Liang Yu  Hsi‐Hsun Chen  Jau‐Min Wong  Paul Matsudaira  Peter T. C. So  George Barbastathis
Abstract:Optical sectioning techniques offer the ability to acquire three‐dimensional information from various organ tissues by discriminating between the desired in‐focus and out‐of‐focus (background) signals. Alternative techniques to confocal, such as active structured illumination, exist for fast optically sectioned images, but they require individual axial planes to be imaged consecutively. In this article, an imaging technique (THIN), by utilizing active Talbot illumination in 3D and multiplexed holographic Bragg filters for depth discrimination, is demonstrated for imaging in vivo 3D biopsy without mechanical or optical axial scanning.
Keywords:3D wide field Microscopy  optical sectioning  holographic Bragg filters  Talbot effect
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号