DNA sensor by using electrochemiluminescence of acridinium ester initiated by tripropylamine |
| |
Authors: | Yi He Hao Zhang Ying Chai Hua Cui |
| |
Institution: | (1) CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, 230026, People’s Republic of China; |
| |
Abstract: | It was found that tripropylamine (TPA) could be used as a coreactant to initiate the electrochemiluminescence (ECL) of acridinium
NHS ester (AE NHS) labels attached to DNA. The radicals generated in the electro-oxidation process of TPA reacted with AE
NHS to form the excited N-methylacridone, giving rise to light emission. The AE/TPA ECL system was for the first time used as the detection system
for developing an ECL-based DNA sensor. In the protocol, streptavidin-modified gold nanoparticles were firstly immobilized
onto a thiol-treated gold electrode. The streptavidin could specifically interact with the biontinylated capture DNA. Afterwards,
the target DNA and the AE-labeled report DNA were conjugated onto the electrode step by step due to the hybridization reactions,
and a sandwich-type sensor was fabricated. The ECL signals of the sensor were obtained under pulse potential condition in
alkaline solution containing 50.0 mmol L−1 TPA. Under optimized experimental conditions, the linear range of the DNA sensor for the determination of the target DNA
was from 5.0 × 10−15 to 5.0 × 10−12 mol L−1. The detection limit (S/N = 3) was 3.0 × 10−15 mol L−1. Moreover, the sensor could specifically recognize the target DNA against one base-pair mismatched sequences, two base-pair
mismatched sequences, and the noncomplementary sequences. It is of great application potential in clinic analysis. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|