| SPR免疫竞争法研究小分子吗啡与其抗体间作用的动力学特征 |
| |
| 引用本文： | 陈受惠,崔小强,杨帆,倪永年,杨秀荣. SPR免疫竞争法研究小分子吗啡与其抗体间作用的动力学特征[J]. 高等学校化学学报, 2003, 24(10): 1770-1774 |
| |
| 作者姓名： | 陈受惠 崔小强 杨帆 倪永年 杨秀荣 |
| |
| 作者单位： | 1. 中国科学院长春应用化学研究所电分析化学国家重点实验室, 长春 130022;2. 南昌大学化学系, 南昌 330047 |
| |
| 基金项目： | 国家自然科学基金 (批准号 :2 0 0 75 0 2 7)，国家重点基础研究规划项目 (批准号 :2 0 0 1CB5 10 2 )资助 |
| |
| 摘 要： | 表面等离子体共振技术(SPR)主要应用于生物大分子相互作用的研究,本文采用溶液竞争法,测定了小分子吗啡与其抗体作用的结合常数K1,并计算了吗啡抗体与吗啡化牛血清白蛋白的结合常数K2.证明了多抗对抗原的亲和力较单抗大,并表明大分子蛋白质的存在对抗体与待测物的结合有阻碍作用.
|
| 关 键 词： | 表面等离子体共振(SPR) 动力学 吗啡 溶液竞争法 免疫分析 |
| 文章编号： | 0251-0790(2003)10-1770-05 |
| 收稿时间： | 2002-11-05 |
Kinetic Characteristics of the Interaction Between Morphine and Its Antibody by SPR Competitive Immunoassay |
| |
| CHEN Shou-Hui ,,CUI Xiao-Qiang ,YANG Fan ,NI Yong-Nian ,YANG Xiu-Rong. Kinetic Characteristics of the Interaction Between Morphine and Its Antibody by SPR Competitive Immunoassay[J]. Chemical Research In Chinese Universities, 2003, 24(10): 1770-1774 |
| |
| Authors: | CHEN Shou-Hui CUI Xiao-Qiang YANG Fan NI Yong-Nian YANG Xiu-Rong |
| |
| Affiliation: | 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academyof Sciences, Changchun 130022, China;2. Department of Chemistry, Nanchang University, Nanchang 330047, China |
| |
| Abstract: | It is impossible for surface plasmon resonance to measure directly the binding kinetics between a low-molecular-weight analyte interacting and its immobilized binding partner. Solution competition method was applied to the kinetic study of the interaction between morphine and its antibody. The affinity constant between the antibody of morphine and morphine-BSA immobilized on the sensor chip was also obtained. The result showed that the affinity of polyclonal antibody is stronger than that of monoclonal antibody. And it also indicated that the protein combined with the analyte affected the binding of antibody to antigen. |
| |
| Keywords: | Surface plasmon resonance(SPR) Kinetics Morphine Solution competition method Immunoassay |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《高等学校化学学报》浏览原始摘要信息 |
|
点击此处可从《高等学校化学学报》下载全文 |
