Magnetic beads-based electrochemical immunosensor for detection of pseudorabies virus antibody in swine serum |
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| Authors: | Fang Li Rui Zhou Kaihong Zhao Huanchun Chen Yonggang Hu |
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| Institution: | 1. School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China;2. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China;3. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: | A novel magnetic electrochemical immunosensor has been developed for the detection of pseudorabies virus antibody in swine serum. The magnetic glass carbon electrode was fabricated to manipulate magnetic beads for the direct sensing applications. Magnetic beads were employed as the platforms for the immobilization and immunoreaction process, and gold nanoparticles were chosen as electroactive labels for the electrochemical detection. The parameters concerning the assay strategy were carefully investigated. Under the optimal conditions, the linear response range of pseudorabies virus antibody dilution ratio (standard positive serum) was 1:250 to 1:1000 with a detection limit of 1:1000. Finally, this developed immunoassay method was successfully applied in the detection of pseudorabies virus antibody in swine serum, and had a good diagnostic accordance in comparison with ELISA. |
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