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An alcohol oxidase-based electrochemical sensor for the rapid determination of lower alcohols |
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| Authors: | V A Alferov M G Zaitsev O N Ponomareva T A Kuznetsova T V Rogova A N Reshetilov |
| Institution: | 1.Faculty of Physical Sciences,Tula State University,Tula,Russia;2.Skryabin Institute of Biochemistry and Physiology of Miscroorganisms,Russian Academy of Sciences,Pushchino, Moscow oblast,Russia |
| Abstract: | The analytical and performance characteristics are compared for biosensors of cell and flow injection types with bioselective membranes based on four yeast alcohol oxidases. The recognition element based on the alcohol oxidase ifrom the methylotrophic yeast Hansenula polymorpha NCYC 495 ln is shown to be not worse and in many parameters superior to the elements based on commercially available preparations of alcohol oxidases. The detection limits for alcohols by biosensors of flow and cell types make 0.01–0.015 mM. |
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