Variable Volume Fed-Batch Fermentation for Nisin Production by <Emphasis Type="Italic">Lactococcus lactis</Emphasis> subsp<Emphasis Type="Italic">. lactis W28</Emphasis> |
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Authors: | Zhaoliang Wu Lin Wang Yingjun Jing Xueliang Li Yanli Zhao |
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Institution: | (1) Department of Bioengineering, Hebei University of Technology, Tianjin, 300132, China |
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Abstract: | A feeding technology that was suitable for improving the nisin production by Lactococcus lactis subsp. lactis W28 was established. The effects of initial sucrose concentration (ISC) in the fermentation broth, feeding time, and feeding
rate on the fermentation were studied. It was observed that a fed-batch culture (ISC = 10 g l−1) with 100 ml sucrose solution (190 g l−1) being evenly fed (9–10 ml h−1) into the fermenter after 3-h fermentation gave the best performance in terms of biomass and nisin yield. Under these conditions,
the total biomass and the total nisin yield were approximately 23% and 51% higher than those in batch fermentation, respectively.
When the sucrose concentration was controlled at 5–10 g l−1 in variable volume intermittent fed-batch fermentation (VVIF) with ISC = 10 g l−1, the total biomass and the total nisin yield were 29% and 60% above those in batch fermentation, respectively. The VVIF proved
to be effective to eliminate the substrate inhibition by maintaining sucrose at appropriate levels. It is also easy to be
scaled up, since various parameters involved in industrial production were taken into account. |
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Keywords: | Nisin Fed-batch Fermentation Lactic acid bacteria Biotechnology |
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