New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches |
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Authors: | Davy Guillarme Josephine Ruta Serge Rudaz Jean-Luc Veuthey |
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Institution: | (1) School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d’Yvoy 20, 1211 Geneva 4, Switzerland |
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Abstract: | Recent developments in chromatographic supports and instrumentation for liquid chromatography (LC) are enabling rapid and
highly efficient separations. Various analytical strategies have been proposed, for example the use of silica-based monolithic
supports, elevated mobile phase temperatures, and columns packed with sub-3 μm superficially porous particles (fused core)
or with sub-2 μm porous particles for use in ultra-high-pressure LC (UHPLC). The purpose of this review is to describe and
compare these approaches in terms of throughput and resolving power, using kinetic data gathered for compounds with molecular
weights ranging between 200 and 1300 g mol−1 in isocratic and gradient modes. This study demonstrates that the best analytical strategy should be selected on the basis
of the analytical problem (e.g., isocratic vs. gradient, throughput vs. efficiency) and the properties of the analyte. UHPLC
and fused-core technologies are quite promising for small-molecular-weight compounds, but increasing the mobile phase temperature
is useful for larger molecules, for example peptides. |
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