腺病毒介导的wtp53基因对五种癌细胞系的生长抑制作用

构建重组腺病毒载体 Ad CMVp53 ,将 wtp53分别导入 PG(人肺巨细胞瘤细胞 )、CAE(人结肠癌细胞 )、HCT(人结肠癌细胞 )、He La(人宫颈癌细胞 )及 BEL74 0 2 (人肝癌细胞 )五种癌细胞中 ,测定 Ad CMVp53对癌细胞的半抑制浓度 ( IC5 0 )和细胞生长曲线 ,分析不同组织癌细胞对 Ad CMVp53的敏感程度 .结果表明 ,Ad CMVp53对癌细胞具有与病毒剂量相关的明显致死作用 ,但不同细胞对腺病毒剂量的敏感程度有差异 ,PG、CAE和BEL74 0 2三个细胞系较为敏感 ,其 IC5 0 低于 1 5MOI(重复感染率 ,multipicity of infection) ;而 He La和 HCT细胞需要较高的病毒剂量 ,IC5 0 分别为 1 0 0 MOI和 80 0 MOI.

CROWTH SUPPRESSION OF FIVE CANCER CELLS BY ADENOVIRUS-MEDIATED WTP53 GENE

A recombinant adenovirus (AdCMVp53) was constructed. Five human cancer cells PG (lung carcinoma), CAE (colon carcinoma), HCT (colon carcinoma), HeLa (cervix carcinoma) and BEL7402 (liver carcinoma) were infected with AdCMVp53, and the cell growth inhibitory effects of AdCMVp53 were determined by counting cell number. According to IC 50 (doses required to kill 50% of cells) and the cell growth curves, dose dependent death induced by wtp53 was observed in the cells infected by AdCMVp53 in different cell lines. Three cell lines, i.e. PG, CAE and BEL7402, of which IC 50 were below 15MOI (multiplicity of infection), were more sensitive to doses of AdCMVp53 than HeLa and HCT cell, of which IC 50 were 100MOI and 80MOI respectively. The results showed a possible therapeutic strategy in PG (lung cancer), CAE (colon carcinoma) and BEL7402 (hepatoma).