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Establishing a protocol for trace element determinations in serum samples from healthy elderly population in São Paulo city, SP, Brazil |
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| Authors: | M. Saiki N. M. Sumita O. Jaluul I. F. Sobreiro W. Jacob Filho M. B. A. Vasconcellos |
| Affiliation: | 28301. Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, Av. Prof. Lineu Prestes 2242, 05508-000 S?o Paulo, Brazil 28302. Central Laboratory Division and Laboratories of Medical Investigation, Hospital das Clínicas, Faculdade de Medicina,USP, Rua Dr Enéas de Carvalho Aguiar 155, 05403-010 S?o Paulo, Brazil 28303. Faculdade de Medicina, Universidade de S?o Paulo, Rua Dr Enéas de Carvalho Aguiar 155, 05403-010 S?o Paulo, Brazil 28304. Central Laboratory Division and Laboratories of Medical Investigation, Hospital das Clínicas, Faculdade de Medicina,USP, Rua Dr Enéas de Carvalho Aguiar 155, 05403-010 S?o Paulo, Brazil 28305. Faculdade de Medicina, Universidade de S?o Paulo, Rua Dr Enéas de Carvalho Aguiar 155, 05403-010 S?o Paulo, Brazil 28306. Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, Av. Prof. Lineu Prestes 2242, 05508-000 S?o Paulo, Brazil |
| Abstract: | Summary In this study a protocol for blood serum analysis was defined and the concentrations for Br, Ca, Cl, Fe, Na, Rb, Se and Zn were obtained by instrumental neutron activation analysis. Blood samples were collected from healthy elderly volunteers who were selected based on the SENIEUR protocol. Contamination of blood by the collection procedure was also evaluated and found negligible. The serum was separated by centrifugation, then freeze-dried and analyzed. Most of results obtained were within the acceptable value ranges used by physicians for normal population. The certified reference material, NIST SRM 1566b Oyster Tissue was analyzed for quality control. |
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