| 毛细管电泳-电化学检测法测定饲料中的磺胺类药物 |
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| 引用本文： | 张寅艳,靳淑萍,李自成,杨志勇,王清江,何品刚,方禹之.毛细管电泳-电化学检测法测定饲料中的磺胺类药物[J].分析试验室,2010,29(3). |
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| 作者姓名： | 张寅艳 靳淑萍 李自成 杨志勇 王清江 何品刚 方禹之 |
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| 作者单位： | 华东师范大学,上海,200062 |
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| 基金项目： | 中国自然科学基金(20575022);;上海市科委(08142201400)项目资助 |
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| 摘 要： | 采用毛细管电泳-电化学检测法(CE-ED),对饲料中的6种磺胺类药物磺胺脒、磺胺二甲嘧啶、磺胺甲嘧啶、磺胺二甲氧嘧啶、磺胺嘧啶、磺胺甲恶唑进行了分离和测定。分别考察了工作电极电位、运行缓冲液的pH和浓度、分离电压和进样时间等实验参数对实验结果的影响。在优化的实验条件下,以直径300μm的碳圆盘电极为工作电极,检测电位为0.95 V(vs.SCE),在30 mmol/L硼砂-KH2PO4(pH7.6)的运行缓冲溶液中,6个分析物能够在16 min内实现很好的基线分离,被测物浓度与峰电流在3个数量级呈良好的线性,检出限(S/N=3)范围0.08～0.20μg/mL。该方法已应用于实际样品的分析。
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| 关 键 词： | 毛细管电泳 电化学检测 饲料 磺胺 |
Determination of sulfonamides in feed by capillary electrophoresis with electrochemical detection |
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| ZHANG Yin-yan,JIN Shu-ping,LI Zi-cheng,YANG Zhi-yong,WANG Qing-jiang,HE Pin-gang,FANG Yu-zhi.Determination of sulfonamides in feed by capillary electrophoresis with electrochemical detection[J].Chinese Journal of Analysis Laboratory,2010,29(3). |
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| Authors: | ZHANG Yin-yan JIN Shu-ping LI Zi-cheng YANG Zhi-yong WANG Qing-jiang HE Pin-gang FANG Yu-zhi |
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| Institution: | East China Normal University;Shanghai 200062 |
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| Abstract: | A method based on capillary electrophoresis with electrochemical detection(CEED) has been developed for the separation and determination of six sulfonamides (sulfaguanidine,sulfamethazine,sulfamerazine,sulfadimethoxine,sulfadiazine,and sulfamethoxazole) in feed.The effects of working electrode potential,pH and concentration of running buffer,separation voltage,and injection time on CE-ED were investigated.Under the optimum conditions,the six analytes could be separated within 16 min at a separation voltage ... |
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| Keywords: | Sulfonamides Capillary electrophoresis Electrochemical detection Feed |
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