Membrane orientation of the Na,K-ATPase regulatory membrane protein CHIF determined by solid-state NMR |
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Authors: | Franzin Carla M Teriete Peter Marassi Francesca M |
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Institution: | Burnham Institute for Medical Research, La Jolla, CA 92037, USA. |
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Abstract: | Corticosteroid hormone-induced factor (CHIF) is a major regulatory subunit of the Na,K-ATPase, and a member of an evolutionarily conserved family of membrane proteins that regulate the function of the enzyme complex in a tissue-specific and physiological-state-specific manner. Here we present the structure of CHIF oriented in the membrane, determined by solid-state NMR orientation-dependent restraints. Because CHIF adopts a similar structure in lipid micelles and bilayers, it is possible to assign the solid-state NMR spectrum measured for (15)N-labeled CHIF in oriented bilayers from the structure determined in micelles, to obtain the global orientation of the protein in the membrane. |
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