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Chromato-photocolorimetric determination of tselanid (lanatoside C)
Authors:M. B. Mavlyanova  D. M. Popov
Abstract:Methods have been developed for the chromato-colorimetric determination of tselanid (lanatoside C) as such and in tablets and solutions. The chloroform-methanol-water (80:19:1) solvent system was used. Chromatography was performed by the ascending method on Silufol UV-254 plates. The revealing agent was a 1% solution of vanillin in 10% perchloric acid. Quantitative determination was performed photocolorimetrically by the reaction with sodium picrate. The methods developed have given accurate results correlating with those of high-performance liquid chromatography and the biological method. The relative error of this determination does not exceed ±4% [1].Tashkent Pharmaceutical Institute. All-Union Scientific-Research Institute of Pharmacy, Moscow. Translated from Khimiya Priodnykh Soedinenii, No. 3, pp. 332–336, May–June, 1986.
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