一株高产抗菌活性物质链霉菌的初步分类鉴定

对一株土壤中筛选出的可以产抗菌活性物质的链霉菌进行了研究，测定了该菌的抗菌谱，发现该菌株对多种植物病原性真菌及细菌有拮抗作用，但对植物病原性真菌的抑制效果低于植物病原性细菌.通过形态特征、培养特征、生理生化特性及抑菌谱等系列比较，发现该链霉菌菌株与链霉菌属的黑胡桃链霉菌(Streptomyces nogalater)的特征基本相符；通过16S?rDNA序列比对，发现该菌株16S?rDNA与黑胡桃链霉菌的16S?rDNA同源性达99％.根据多项分类原则和系统进化树的构建分析，将该菌株暂归入黑胡桃链霉菌类.

Preliminary classification and identification of Streptomyces strain 45#

The Streptomyces strain 45 #, which was isolated from soil, can produce broad-spectrum antimicrobial substances with high activities. Analysis on these antimicrobial substances showed that they have notable antagonistic effects on phytopathogens as well as some bacteria, but unconspicuous effects on fungi comparatively. A similarity of basic properties between Streptomyces strain 45 # and Streptomyces nogalater was proved by a series of comparison of the morphological and cultural characteristics, and the physiological and biochemical properties. The results exhibited that the homology of 16S rDNA between these two strains reached 99%. A phylogenetic tree was constructed by comparing with some correlative published 16S rDNA sequences. According to the multi-classification principle and the phylogenetic analysis, the Streptomyces strain 45 # should be nomenclatured as Strptomyces nogalater.