| STUDIES ON THE RNA POLYMERASES FROM THE CELL NUCLEI OF VICIA FABA L.LEAVES |
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| 作者姓名： | 白永延 曹湘玲 唐惕 毛慧珠 |
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| 作者单位： | Laboratory of Cell Physiology，Shanghai Institute of Plant Physiology，Academia Sinica，Laboratory of Cell Physiology，Shanghai Institute of Plant Physiology，Academia Sinica，Laboratory of Cell Physiology，Shanghai Institute of Plant Physiology，Academia Sinica，Laboratory of Cell Physiology，Shanghai Institute of Plant Physiology，Academia Sinica |
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| 摘 要： | Isolated cell nuclei from young leaves of Vicia faba L. have a high level of RNA polymorose activity and can serve as a good source for purification of higher plant RNA polymerases. The nuclei were lysed in a solution of high (NH_4)_2SO_4 concentration and the lysate was subjected to sonication to liberate RNA polymerases, which then were fractionated on DEAE-cellulose and DEAE-Sephadex columns. Three enzyme peaks were detected in the eluate fractions. According to their sensitivity to α-amanitin they are the RNA polymerases Ⅰ, Ⅱ and Ⅲ. Divalent cation Mn or Mg is essential for the enzyme activity. The Mn optimum concentration is 3mM and the Mg optimum concentration is 5mM for the RNA polymerases Ⅰ and Ⅱ. Calf thymus DNA and the DNA of Vicia faba have equal efficiency as template for RNA synthesis by these enzymes.
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