| 结晶紫共振光散射法测定脱氧核糖核酸 |
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| 引用本文： | 刘晨,胡灏,陈小明.结晶紫共振光散射法测定脱氧核糖核酸[J].分析试验室,2001,20(2):30-33. |
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| 作者姓名： | 刘晨 胡灏 陈小明 |
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| 作者单位： | 湘潭大学化学化工学院, |
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| 基金项目： | 湖南省自然科学基金!项目 ( 0 0 JJY2 0 84 ) |
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| 摘 要： | 研究了三苯甲烷类碱性染料结晶此与DNA作用的共振光散射光谱，在pH9.2-10.5的范围内，加入DNA导致结晶紫共振光散射增强，在512nm处，存在一共振光散射增强峰，其强度与DNA的浓度呈线性关系，据此建立了一种测定DNA的菜振光散射法，方法的线性范围为0－900ng/mL，检出限为5.02mg/mL.已用于混合样品中的DNA的测定.
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| 关 键 词： | 结晶紫 共振光散射法 DNA 测定方法 |
| 文章编号： | 1000-0720(2001)02-0030-04 |
Determination of Deoxyribonucleic Acid with Crystal Violet by Resonance Light Scattering Method |
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| LIU Chen,HU Hao and CHEN Xiao ming.Determination of Deoxyribonucleic Acid with Crystal Violet by Resonance Light Scattering Method[J].Chinese Journal of Analysis Laboratory,2001,20(2):30-33. |
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| Authors: | LIU Chen HU Hao and CHEN Xiao ming |
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| Abstract: | Resonance light scattering (RLS) spectra of crystal violet withDNA was studied. The RLS of crystal violet was greatly enhanced by DNA in the range of pH 9.2～10.5. A resonance light scattering peak at 512nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0～900 ng/mL with the detection limit of 5.02 ng/mL. Compared with other methods, this method has larger detective wavelength, which can reduce the interference of background and increase the intensity of resonance light scattering. |
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| Keywords: | Crystal violet Resonance light scattering method DNA |
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