Characteristic neutral loss of CH3CHO from Thr‐containing sodium‐associated peptides |
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Authors: | Huixin Wang Bing Wang Zhonglin Wei Yanwei Cao Xinshu Guan Xinhua Guo |
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Institution: | College of Chemistry, Jilin University, Changchun, China |
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Abstract: | A characteristic neutral loss of 44 Da is observed in the MS/MS spectra of Thr‐containing sodiated peptides. A combination of tandem mass spectrometry and quantum chemical calculations calculated at the B3LYP/6‐311G (d, p) level of ab initio theory is used to elucidate this fragmentation pathway. The high resolution mass spectrometry data indicate this neutral loss is acetaldehyde lost from the side chain of Thr rather than CO2. The intensity of this neutral loss can be enhanced when Thr residue is far from the C‐terminus and when the C‐terminus is esterified as well. The mechanism of the acetaldehyde loss is proposed to adopt a McLafferty‐type rearrangement reaction, which involves a proton transfer from the hydroxyl of Thr side chain to its C‐terminal neighboring carbonyl oxygen inducing the cleavage of the Ca–Cβ bond. This mechanism is further supported by examining the fragmentation of a GT(tBu)G + Na]+ peptide derivative and by comparing the product ion spectra of M + Na‐44]+ of GTGA + Na]+ with M + Na]+ of GGGA + Na]+. A similar neutral loss of HCHO can also be detected in Ser‐containing peptides. Our computational results reveal that the most stable GTG + Na]+ ion is present as a tridentate charge‐solvated structure and the dissociation leading to the 44 loss is dynamically and energetically favorable. Copyright © 2015 John Wiley & Sons, Ltd. |
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Keywords: | sodium‐associated peptide threonine 44 Da neutral loss McLafferty‐type rearrangement charge‐remote |
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