Direct chemical profiling of olive (Olea europaea) fruit epicuticular waxes by direct electrospray‐ultrahigh resolution mass spectrometry |
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| Authors: | Stefania Vichi Nuria Cortés‐Francisco Agustí Romero Josep Caixach |
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| Affiliation: | 1. Food Science and Nutrition Department, XaRTA (Catalonian Reference Network on Food Technology), University of Barcelona, S.ta Coloma de Gramenet, Spain;2. Olive Production, Oil Processing and Nut Trees, IRTA, Constantí (Tarragona), Spain;3. Mass Spectrometry Laboratory/Organic Pollutants, IDAEA‐CSIC, Barcelona, Spain |
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| Abstract: | In the present paper, an electrospray ionization (ESI)‐Orbitrap method is proposed for the direct chemical profiling of epicuticular wax (EW) from Olea europaea fruit. It constitutes a rapid and efficient tool suitable for a wide‐ranging screening of a large number of samples. In a few minutes, the method provides a comprehensive characterization of total EW extracts, based on the molecular formula of their components. Accurate mass measurements are obtained by ultrahigh resolution mass spectrometry, and compositional restrictions are set on the basis of the information available from previous studies of olive EW. By alternating positive and negative ESI modes within the same analysis, complementary results are obtained and a wide range of chemical species is covered. This provides a detailed compositional overview that otherwise would only be available by applying multiple analytical techniques. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | ultrahigh resolution mass spectrometry (UHRMS) direct injection profiling epicuticular waxes olive |
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