Real-time assay of immobilized tannase with a stopped-flow conductometric device |
| |
| Authors: | Chang Fu-Shiang Chen Po-Chung Chen Richie L C Lu Fu-Ming Cheng Tzong-Jih |
| |
| Institution: | Department of Bio-Industrial Mechatronics Engineering, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan. |
| |
| Abstract: | A stopped-flow manifold was developed to assay and characterize immobilized tannase (EC 3.1.1.20). The immobilized enzyme reactor was inserted within the tube-type electrode pair (cell constant=103.2 cm(-1)) for a real-time conductometric measurement. Tris buffer (2 mM, pH=7.0) was used as the carrier for sensitivity improvement. The activities and kinetic parameters (Km values) for propyl gallate, methyl gallate and tannic acid were investigated. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
