Simultaneous determination of trimipramine and its major metabolites by high-performance liquid chromatography |
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| Authors: | A A Gulaid G A Jahn C Maslen M J Dennis |
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| Affiliation: | Biopharmacy Department, Rh?ne-Poulenc Rorer, Ltd., Dagenham, Essex, U.K. |
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| Abstract: | Trimipramine is a tricyclic antidepressant drug often assayed by gas chromatographic or gas chromatographic-mass spectrometry techniques. A high-performance liquid chromatographic method with electrochemical detection is described for the assay of trimipramine and its major metabolites, monodesmethyltrimipramine and 2-hydroxytrimipramine, in plasma. The method is sensitive, accurate and robust and thus suitable for routinely assaying samples following single doses of trimipramine to man. The assay was applied to plasma samples obtained following a single 50-mg dose of trimipramine to healthy volunteers. |
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