Modifier effects on packed and capillary columns in supercritical fluid chromatography |
| |
Authors: | S Schmidt L G Blomberg E R Campbell |
| |
Institution: | (1) Department of Analytical Chemistry, University of Stockholm, Arrhenius Laboratory, S-106 91 Stockholm, Sweden;(2) Lee Scientific, Inc., 84123 Salt Lake City, UT, USA |
| |
Abstract: | Summary The separation of polar thermally labile solutes is one of the potentially most rewarding fields of SFC application. A presupposition for such applications is, however, mobile phases having relatively high solvent strengths. A promising approach to achieve this is the use of mobile phases consisting of carbon dioxide with a polar additive. In this work, the chromatographic effects of different concentrations of an additive, isopropanol, in carbon dioxide have been studied on capillary and packed columns. A series of antibiotics was used as test substances. Best results were obtained with carbon dioxide/8% isopropanol as mobile phase on a capillary column coated with a cyanopropyl-substituted polysiloxane stationary phase. |
| |
Keywords: | Supercritical fluid chromatography Mixed mobile phases Capillary and packed columns Antibiotics |
本文献已被 SpringerLink 等数据库收录! |
|