| 层层自组装技术制备PDDA-多壁碳纳米管-胆碱生物传感器 |
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| 引用本文： | 陈丽丽,王会才,秦霞,王新胜,赵紫霞,苗智颖,赵薇,单苗苗,陈强. 层层自组装技术制备PDDA-多壁碳纳米管-胆碱生物传感器[J]. 分析化学, 2010, 38(3). DOI: 10.3724/SP.J.1096.2010.00337 |
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| 作者姓名： | 陈丽丽 王会才 秦霞 王新胜 赵紫霞 苗智颖 赵薇 单苗苗 陈强 |
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| 作者单位： | 南开大学生命科学学院,生物活性材料教育部重点实验室,天津,300071;南开大学生命科学学院,生物活性材料教育部重点实验室,天津,300071;南开大学生命科学学院,生物活性材料教育部重点实验室,天津,300071;南开大学生命科学学院,生物活性材料教育部重点实验室,天津,300071;南开大学生命科学学院,生物活性材料教育部重点实验室,天津,300071;南开大学生命科学学院,生物活性材料教育部重点实验室,天津,300071;南开大学生命科学学院,生物活性材料教育部重点实验室,天津,300071;南开大学生命科学学院,生物活性材料教育部重点实验室,天津,300071;南开大学生命科学学院,生物活性材料教育部重点实验室,天津,300071 |
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| 基金项目： | 国家自然科学基金(Nos.30870665,30772746);;天津市自然科学基金(No.08JCZDJC20600)资助项目 |
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| 摘 要： | 以PDDA分散多壁碳纳米管(MWCNTs),制得PDDA-MWCNTs悬浮液,采用层层自组装技术将PDDA-MWCNTs与胆碱氧化酶(ChOx)交替组装在铂(Pt)电极上,最后滴加Nafion制备抗干扰膜,构建了电流型胆碱生物传感器。实验结果表明,构建的复合酶膜(PDDA-MWCNTs/ChOx)n对底物胆碱的催化电流随着组装层数增多而明显增大,酶膜组装6层时最优。此传感器在5×10-6～2.5×10-4mol/L浓度范围内对胆碱有良好线性范围,响应时间为6.6s;灵敏度为21.97μA/mmol;检出限为2×10-6mol/L(S/N=3),RSD<5%,且传感器抗干扰能力强、稳定性好。
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| 关 键 词： | 多壁碳纳米管 聚二烯丙基二甲基氯化铵 胆碱生物传感器 层层自组装技术 |
Amperometric Choline Biosensor Based on Dispersion of Multi-walled Carbon Nanotubes in Poly(diallyldimethylammonium chloride) via Layer-by-Layer Assembly Technique |
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| CHEN Li-Li,WANG Hui-Cai,QIN Xia,WANG Xin-Sheng,ZHAO Zi-Xia,MIAO Zhi-Ying,ZHAO Wei,SHAN Miao-Miao,CHEN Qiang. Amperometric Choline Biosensor Based on Dispersion of Multi-walled Carbon Nanotubes in Poly(diallyldimethylammonium chloride) via Layer-by-Layer Assembly Technique[J]. Chinese Journal of Analytical Chemistry, 2010, 38(3). DOI: 10.3724/SP.J.1096.2010.00337 |
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| Authors: | CHEN Li-Li WANG Hui-Cai QIN Xia WANG Xin-Sheng ZHAO Zi-Xia MIAO Zhi-Ying ZHAO Wei SHAN Miao-Miao CHEN Qiang |
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| Affiliation: | The Key Laboratory of Bioactive Materials;Ministry of Education;College of Life Science;Nankai University;Tianjin 300071 |
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| Abstract: | Poly( diallyldimethylammonium chloride) ( PDDA) was chosen to disperse multi-walled carbon nanotubes( MWCNTs) to prepare the stable PDDA-MWCNTs aqueous dispersion. Then,the positively charged PDDA-MWCNTs composite and negatively charged choline oxidase( ChOx) were employed to fabricate multi-layer films on platinum( Pt) electrodes by layer-by-layer self-assembly technique,the anti-interferential film of Nafion was dropped at the end of the last multilayer films. The results showed that MWCNTs were evenly di... |
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| Keywords: | Multi-walled carbon nanotubes Poly(diallyldimethylammonium chloride Choline biosensor Layer-by-layer assembly technique |
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