| 光学免疫传感器中芯片的制备 |
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| 引用本文： | 宋保栋,施汉昌,何苗,张凡,郭彬彬.光学免疫传感器中芯片的制备[J].清华大学学报(自然科学版)网络.预览,2007(6). |
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| 作者姓名： | 宋保栋 施汉昌 何苗 张凡 郭彬彬 |
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| 作者单位： | 清华大学环境科学与工程系 北京100084(宋保栋，施汉昌，何苗，张凡)，中国矿业大学 北京100083(郭彬彬) |
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| 基金项目： | 国家“八六三”高技术项目(2005AA641040) |
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| 摘 要： | 为满足光学免疫传感器在环境监测和食品卫生检测领域中应用的要求,研究了一种免疫芯片制备方法,并用平面波导型荧光免疫传感器对免疫芯片的性能进行了评价。该方法可以将小分子物质固定到玻璃基底上。试验结果表明:该芯片能够有效抑制蛋白质的非特异性吸附,并对目标物质抗体的响应符合Logistic方程;修饰基片的氨基葡聚糖的相对分子质量和氨基摩尔比都影响免疫芯片对抗体响应灵敏度;平行测定20次的信号标准偏差小于5%,测定后芯片性能没有明显下降。
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| 关 键 词： | 环境监测 免疫芯片 光学免疫传感器 氨基葡聚糖 非特异性吸附 |
Optical immuno-sensors chip for enviro-nmental monitoring and food security detection |
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| Authors: | SONG Baodong SHI Hanchang HE Miao ZHANG Fan GUO Binbin |
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| Institution: | SONG Baodong1,SHI Hanchang1,HE Miao1,ZHANG Fan1,GUO Binbin2 |
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| Abstract: | An optical immuno-sensor chip was developed for environmental monitoring and detection of food security.The planar waveguide fluorescence immuno-sensor was used to evaluate the chip performance.This chip immobilizes small amount of molecular substances onto a glass substrate.The test results show that chips prepared have little non-specific adsorption to proteins and the response to various antibody concentrations fitted the Logistic formula.The molecular mass and amino acid content of the amino-dextran used in the chip influences the chip sensitivity to antibodies.The signal standard deviation was less than 5% in 20 parallel tests.The chip performance did not deteriorate with time. |
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| Keywords: | environmental monitoring immuno-chip optical immuno-sensors amino-dextran non-specific adsorption |
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