Determination of ascorbic acid in pharmaceutical dosage forms and urine by means of an oscillatory reaction system using the pulse perturbation technique |
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Authors: | Nataša Pejić Slavica Blagojević Slobodan Anić Ljiljana Kolar-Anić |
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Institution: | (1) Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia;(2) Faculty of Physical Chemistry, University of Belgrade, Studentski trg 12–16, P.O. Box 137, 11001 Belgrade, Serbia |
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Abstract: | A simple and reliable method for the determination of ascorbic acid (AA) is proposed and validated. It is based on potentiometric
monitoring of the concentration perturbations of an oscillatory reaction system in a stable nonequilibrium stationary state
close to the bifurcation point. The response of the Bray–Liebhafsky (BL) oscillatory reaction as a matrix, to the perturbation
by different concentrations of AA, is followed by a Pt electrode. The linear relationship between maximal potential shift
and the logarithm of the amount of AA is obtained between 0.01 and 1.0 μmol. The sensitivity of the proposed method (as the
limit of detection) is 0.009 μmol and the method has excellent sample throughput (30 samples per hour). The procedure was
used for AA determination in pharmaceutical formulations and urine. The results are in agreement with those obtained using
the official method. Some aspects of the possible mechanism of AA action on the BL oscillating chemical system are discussed. |
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Keywords: | Determination of ascorbic acid Perturbation technique Bray-Liebhafsky oscillatory reaction Pharmaceutical dosage forms Urine |
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