Qualitative and quantitative analysis of gallic acid in Alchemilla vulgaris, Allium ursinum, Acorus calamus and Solidago virga-aurea by chip-electrospray ionization mass spectrometry and high performance liquid chromatography |
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| Authors: | Dumitru Condrat Cristina Mosoarca Alina D. Zamfir Florin Cri?an Maria R. Szabo Alfa X. Lupea |
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| Affiliation: | (1) Northwest German Forest Research Station, Gr?tzelstr. 2, 37079 G?ttingen, Germany;(2) Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Ecology and Forest Inventory, Johann Heinrich von Th?nen-Institute (vTI), A.-M?ller-Stra?e 1, 16225 Eberswalde, Germany;(3) Department of Silviculture and Forest Ecology of the Temperate Zones, Georg-August University G?ttingen, Faculty of Forest Sciences and Forest Ecology, B?sgenweg 1, 37077 G?ttingen, Germany; |
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| Abstract: | This study presents the results obtained from qualitative and quantitative analysis of gallic acid from hydro-alcoholic extracts (methanol, ethanol) of plants from Plantae regnum. Plant qualitative analysis was performed using a novel mass spectrometric (MS) method based on fully automated chip-nanoelectrospray ionization (nanoESI) high capacity ion trap (HCT) while quantitative analysis was carried out by high performance liquid chromatography (HPLC). These methods were applied to Alchemilla vulgaris — common lady’s-mantle (aerial part), Allium ursinum — bear’s garlic (leaves), Acorus calamus — common sweet flag (roots), Solidago virga-aurea — goldenrod (aerial part). Obtained results indicated that methanol extracts (96%, 80%) have a gallic acid content ranging between 0.0011–0.0576 mg mL−1 extract while the ethanol extracts (96%, 60%) exhibit a gallic acid concentration that varies between 0.0010–0.0182 mg mL−1 extract.  |
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