基于体表内部虚拟超谱图的组织结构和成分变化检测

为实现生物组织结构和成分变化的同时检测,提出体表内部虚拟超谱图(virtual internal hyperspec-trum of body surface,VIHBS)概念。在此基础上,设计了由调制光源、平移采集装置和光谱仪构成的VIH-BS采集系统。以猪肉为实验对象,等分为三份,一份为对照,剩余两份分别掺入红色滤光片和注入高散射物质脂肪乳溶液,模拟猪肉组织结构和成分的变化,并距入射光纤/mm处开始每隔0.5mm进行17个点的数据采集。结果表明,调制光源有效地抑制了环境光和光电器件暗电流影响,VIHBS技术在可见光波段实现了红色滤光片定位检测,以及在近红外波段的脂肪乳引起组织成分变化的筛查。这一研究表明VIHBS技术对组织内部结构和成分变化的检测有很大的可行性,随着研究深入有望为人体皮肤异常或疾病的早期诊断和筛查提供新手段。

Study on detection of changes in tissue structure and composition by using virtual internal hyper-spectrum of body surface

Purposed to achieve the detection of changes in tissue structure and composition simultaneously by virtual internal hyper-spectrum of body surface (VIHBS), we designed the VIHBS system containing modulated light, translation stage device for data acquisition and spectrometer. In the present study, pork meat as the experimental subject was equally divided into three pieces. One piece was used for a control study, and the others were separately embedded with red filter and injected into highly scattering intralipid to imitate the changes in tissue structure and composition. And then, data acquisition of 17 points started at source fiber was taken at intervals of 0.5 mm. The results showed that modulated light can effectively inhibit the influences of ambient light and dark current created by optoelectronic devices. In addition, VIHBS technology achieved the locating of red filter by visible-light and the rapid screening of changes in tissue composition caused by intralipid with near infrared light. The study suggests that it is much feasible to detect the changes in tissue structure and composition by VIHBS. And it is concluded that further research will be likely to provide a new method to realize the early diagnosis and screening of human skin.