A validated GC-MS procedure for fast,simple, and cost-effective quantification of glycols and GHB in human plasma and their identification in urine and plasma developed for emergency toxicology |
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Authors: | Markus R Meyer Armin A Weber Hans H Maurer |
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Institution: | (1) Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, 66421 Homburg (Saar), Germany |
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Abstract: | Methods developed for use in emergency toxicology have to be fast and simple. Additionally, such methods should be multi-analyte
procedures because they allow monitoring of analytes of different drug classes in one single body sample. This is important
because often only a limited amount of sample is available and the results have to be reported as fast as possible. Therefore,
we describe the improvement of an existing method published by van Hee at al. The new method is fast and simple and designed
for the simultaneous determination of ethylene glycol, 1,2-propylene glycol, lactic acid, glycolic acid, gamma-hydroxybutyric
acid (GHB), diethylene glycol, triethylene glycol, and tetraethylene glycol in human plasma or urine. A 50-μL aliquot of sample
was deproteinized and 20 μl of the diluted specimen were derivatized using bis-N,O-trimethylsilyl trifluoroacetamide and the catalyst dimethylformamide. After microwave-assisted derivatization, an aliquot
was injected into the gas chromatograph and analyzed with electron ionization mass spectrometry in selective ion monitoring
mode. All compounds are separated within 12 min and detected with a limit of quantification of 0.05 and 0.01 g/L for glycols
and GHB, respectively. Calibration was linear from 0.05 to 1.0 g/L for glycols and 0.01 to 0.2 g/L for GHB. Validation criteria
were shown to be in the required limits with exception of lactic acid. Average analysis time from starting sample preparation
until quantitative plasma results of approximately 35 min was achieved. This turnaround time is considered most appropriate
for emergency cases. |
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