A FRET-based probe with a chemically deactivatable quencher |
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| Authors: | Leriche Geoffray Budin Ghyslain Darwich Zeinab Weltin Denis Mély Yves Klymchenko Andrey S Wagner Alain |
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| Institution: | Laboratory of Functional Chemo-Systems UMR 7199, 74 Route du Rhin, 67401, Illkirch, France. |
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| Abstract: | A new concept of a chemically deactivatable quencher is proposed for a FRET-based probe that turns-on its fluorescence by either an enzymatic cleavage or a chemical reagent (sodium dithionite). This concept allowed us to quantify the caspase-3 cleavage activity in solution and to reveal unreacted probes in cell experiments. |
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