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Immobilization of cellulase using acrylamide grafted acrylonitrile copolymer membranes
Affiliation:1. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China;2. Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing 210042, China;1. Bioprocess Engineering Research Group, Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia;2. School of Nutrition Sciences, University of Ottawa, K1N 6N5, Ontario, Canada;3. Department of Chemical Engineering, Curtin University, 98009, Miri, Sarawak, Malaysia;4. Bioengineering Laboratory, Department of Chemical Engineering, Monash University, Victoria, 3800, Australia;1. Department of Biotechnology, St. Joseph''s College of Engineering, Chennai-600119, India;2. Department of Applied Science and Technology, Anna University, Chennai-600025, India
Abstract:Immobilization of cellulase onto acrylamide grafted acrylonitrile copolymer (PAN) membranes by means of glutaraldehyde has been studied. The bound cellulase was verified by X-ray photoelectron spectroscopy. The activities of free cellulase and immobilized cellulase are determined by measuring the amount of glucose made from carboxymethyl cellulase in the given conditions. Results show that immobilization conditions had some effects on the activity of immobilized cellulase. The immobilized cellulase had a higher Km than free cellulase (0.02 mg/ml) did. The immobilized cellulase had better stability with respect to pH or temperature than free cellulase.
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